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Decoding the Antibody Repertoire

Name: Decoding the Antibody Repertoire
Brand: Springer International Publishing AG
SKU: 16190177
Price: 109.44 EUR
Availability: InStock
Author: Brandon DeKosky
ISBN: 9783319585178

Brandon DeKosky

Língua

Inglês

Livro Livro de capa dura

Código Libristo: 16190177

Editoras Springer International Publishing AG, junho 2017

This thesis outlines the development of the very first technology for high-throughput analysis of pa... Descrição completa

Código Libristo: 16190177

265 b

109.44 €

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This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases.Previous methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells. The techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.